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Need help designing a 194.0 °F bioreactor for cell culture.

stopfortheklopp

Oct 10, 2018
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Oct 10, 2018
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I've been having trouble deciding how to design a 194.0 °F (90ºC) bioreactor for cell culture (not cell culture now but I do need to get to that temperature to take sample). Its supposed to be an incubator, basically a 3mm thick 5.5"x5.5"(14 cm) acrylic box, but I'm not sure If I can reach that temperature by only using 2 20W bulbs (how can I calculate the amount of bulbs needed?) or if I should use a PTC Resistor like [this one.](https://alexnld.com/product/ac-dc-5...MIhLnm4fn63QIVmbrACh1Hug5ZEAYYAiABEgIWtPD_BwE)


The objective is to regulate the temperature inside the box from 60º to 90º C and I'm planning on using a thermistor and an Arduino to regulate the temperature, but I'm convinced there must be an easier way.

Also, would you change the material from acrylic to something else? Would you use a small fan to regulate heat dispersion? And finally, how would you protect the electronics (PCB, Arduino, electrodes) inside the box?

Any help is greatly appreciated.
 

(*steve*)

¡sǝpodᴉʇuɐ ǝɥʇ ɹɐǝɥd
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Jan 21, 2010
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Acrylic gets soft at those temperatures

Get yourself a sous vide machine :)
 
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